[流感病毒杂交血凝素H1-H3基因在大肠杆菌中的构建与表达]
[Construction and expression in Escherichia coli of a gene of hybrid hemagglutinin H1-H3 of influenza virus].
作者信息
Petrenko V A, Kipriianov S M, Mizenko G A, Eroshkin A M, Sivolobova G F, Rukavishnikov M Iu, Akimenko Z A, Boldyrev A N, Kalashnikov V V
出版信息
Mol Biol (Mosk). 1990 Mar-Apr;24(2):408-16.
The hybrid gene of influenza virus hemagglutinin (HA) of the H1-subtype, carrying the sequence coding for the fragment of H3-subtype antigenic site B, was constructed. The product of expression of this gene in E. coli was obtained as a fusion protein with beta-galactosidase. The chimeric protein was shown to retain the antigenic properties of HA of H1-subtype and to interact specifically with antibodies against the synthetic peptide corresponding to the B site fragment of HA of the H3-subtype.
构建了携带H3亚型抗原位点B片段编码序列的H1亚型流感病毒血凝素（HA）杂交基因。该基因在大肠杆菌中的表达产物是以与β-半乳糖苷酶的融合蛋白形式获得的。结果表明，该嵌合蛋白保留了H1亚型HA的抗原特性，并能与针对H3亚型HA的B位点片段合成肽的抗体特异性相互作用。
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