Petrenko V A, Kipriianov S M, Semenova L N, Akimenko Z A, Rukavishnikov M Iu
Mol Biol (Mosk). 1989 May-Jun;23(3):889-98.
The full-length copy of the hemagglutinin gene of influenza virus was inserted into M13 phage DNA. The DNA sequence coding for the hydrophobic prepeptide was removed from the gene by oligonucleotide-directed mutagenesis. The possibilities of expression of the full-length and mutant genes in E. coli were investigated. The beta-galactosidase-hemagglutinin fusion proteins were isolated. The fusion proteins exhibited specific binding to antiviral antibodies. This binding could be competitively inhibited by excess of viral hemagglutinin, demonstrating that these fusion proteins contained antigenic determinants of hemagglutinin.
将流感病毒血凝素基因的全长拷贝插入M13噬菌体DNA中。通过寡核苷酸定向诱变从该基因中去除编码疏水前肽的DNA序列。研究了全长基因和突变基因在大肠杆菌中表达的可能性。分离出β-半乳糖苷酶-血凝素融合蛋白。这些融合蛋白表现出与抗病毒抗体的特异性结合。这种结合可被过量的病毒血凝素竞争性抑制，表明这些融合蛋白含有血凝素的抗原决定簇。
