Rozinov M N, Gol'tsmaĭer T A, Dikarev S D, Gershanovich V N
Mol Gen Mikrobiol Virusol. 1985 Dec(12):12-4.
dsDNA of the influenza virus subtype A/Leningrad/385/80/R (H3N2)-recombinant A/Leningrad/385/80 (H3N2) and RR/8/34 (H1N1) has been synthesized using polyadenylated viral RNA as a template. This dsDNA has been cloned on plasmid pUC19. A clone has been selected harbouring the plasmid with included proximal fragment of hemagglutinin gene that contains the main antigenic determinants. The hybrid plasmid is hybridizable with RNA of the hemagglutinin gene and with oligonucleotide CATGCAAAACCTTCCC that is complementing the sequence coding for the proximal fragment of the mature hemagglutinin.
已使用聚腺苷酸化病毒RNA作为模板合成了甲型流感病毒A/列宁格勒/385/80/R（H3N2）重组体A/列宁格勒/385/80（H3N2）和RR/8/34（H1N1）的双链DNA。该双链DNA已克隆到质粒pUC19上。已选择一个克隆，其携带的质粒包含血凝素基因的近端片段，该片段含有主要抗原决定簇。该杂交质粒可与血凝素基因的RNA以及与成熟血凝素近端片段编码序列互补的寡核苷酸CATGCAAAACCTTCCC杂交。
