Petrenko V A, Kipriianov S M, Semenova L N, Boldyrev A N, Pozdniakov P I, Eroshkin A M, Sivolobova G F
Mol Biol (Mosk). 1990 Sep-Oct;24(5):1230-40.
A new approach to create chimeric genes by directed exchange of oligonucleotide fragments was developed. By oligonucleotide-directed mutagenesis a few deletion mutants of the influenza virus hemagglutinin (HA) gene were obtained. These variants of HA gene contain unique restriction sites in DNA regions coding for the A and B epitopes of the HA molecule. The obtained special vectors may be used for cloning DNA fragments coding for new amino acid sequences in internal sites of the HA gene.
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