Shinohara Takashi, Kato Megumi, Takehashi Masanori, Lee Jiyoung, Chuma Shinichiro, Nakatsuji Norio, Kanatsu-Shinohara Mito, Hirabayashi Masumi
Department of Molecular Genetics and Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
Proc Natl Acad Sci U S A. 2006 Sep 12;103(37):13624-8. doi: 10.1073/pnas.0604205103. Epub 2006 Aug 30.
Spermatogonial transplantation has demonstrated a unique opportunity for studying spermatogenesis and provided an assay for spermatogonial stem cells. However, it has remained unknown whether germ cells that matured in a xenogeneic environment are functionally normal. In this investigation, we demonstrate the successful production of xenogeneic offspring by using spermatogonial transplantation. Rat spermatogonial stem cells were collected from immature testis and transplanted into the seminiferous tubules of busulfan-treated nude mouse testis. Using rat spermatids or spermatozoa that developed in xenogeneic surrogate mice, rat offspring were born from fresh and cryopreserved donor cells after microinsemination with rat oocytes. These offspring were fertile and had a normal imprinting pattern. The xenogeneic offspring production by interspecies germ cell transplantation and in vitro microinsemination will become a powerful tool in animal transgenesis and species conservation.
精原细胞移植为研究精子发生提供了一个独特的机会，并为精原干细胞提供了一种检测方法。然而，在异种环境中成熟的生殖细胞功能是否正常仍不清楚。在本研究中，我们证明了通过精原细胞移植成功产生异种后代。从未成熟睾丸中收集大鼠精原干细胞，并将其移植到经白消安处理的裸鼠睾丸的生精小管中。使用在异种代孕小鼠中发育的大鼠精子细胞或精子，在与大鼠卵母细胞进行显微授精后，从新鲜和冷冻保存的供体细胞中诞生了大鼠后代。这些后代具有生育能力且印记模式正常。通过种间生殖细胞移植和体外显微授精产生异种后代将成为动物转基因和物种保护中的一种强大工具。
