Raju N R, Yaeger M J, Okazaki D L, Lovell K, Koestner A
Department of Pathology, Michigan State University, East Lansing 48824.
Toxicol Pathol. 1990;18(1 Pt 1):18-23. doi: 10.1177/019262339001800103.
Ethylnitrosourea-induced central and peripheral nerve tumors in Sprague-Dawley rats were tested for GFAP (Glial Fibrillary Acidic Protein), S-100 protein, NSE (Neuron Specific Enolase) and Anti-Leu 7 (HNK-1) immunoreactivity utilizing the ABC method (avidin-biotin-complex) for GFAP, S-100 protein and NSE, and the PAP method (peroxidase-antiperoxidase) for Anti-Leu 7. Peripheral nerve neurinomas were consistently positive for S-100 protein and consistently negative for GFAP and Anti-Leu 7. Neurinomas would occasionally exhibit positive staining for NSE (2 of 55 tumors). The staining intensity for S-100 protein varied from strongly positive in differentiated neurinomas to weakly positive in anaplastic tumors. Neoplastic and reactive astrocytes exhibited positive staining for both S-100 protein and GFAP. Variation in the GFAP staining intensity of glial tumors correlated with the degree of differentiation as anaplastic tumors did not stain with the same intensity as their more differentiated counterparts. Oligodendrogliomas exhibited occasional immunoreactivity to S-100 protein (3 of 36 tumors). NSE reactivity in oligodendrogliomas was rarely observed (1 tumor in 36) and immunoreactivity against GFAP or Anti-Leu 7 was consistently absent. Anti-Leu 7 and NSE proved to be of little value in the classification of ENU-induced neural tumors.
利用ABC法（抗生物素蛋白-生物素复合物法）检测胶质纤维酸性蛋白（GFAP）、S-100蛋白和神经元特异性烯醇化酶（NSE），以及利用PAP法（过氧化物酶-抗过氧化物酶法）检测抗Leu 7（HNK-1）免疫反应性，对乙基硝基脲诱导的Sprague-Dawley大鼠中枢和外周神经肿瘤进行检测。外周神经鞘瘤S-100蛋白始终呈阳性，GFAP和抗Leu 7始终呈阴性。神经鞘瘤偶尔会出现NSE阳性染色（55个肿瘤中有2个）。S-100蛋白的染色强度从分化型神经鞘瘤的强阳性到间变性肿瘤的弱阳性不等。肿瘤性和反应性星形胶质细胞对S-100蛋白和GFAP均呈阳性染色。胶质肿瘤中GFAP染色强度的变化与分化程度相关，因为间变性肿瘤的染色强度与其分化程度较高的对应肿瘤不同。少突胶质细胞瘤偶尔对S-一百蛋白有免疫反应性（36个肿瘤中有3个）。少突胶质细胞瘤中很少观察到NSE反应性（36个中有1个肿瘤），并且始终不存在针对GFAP或抗Leu 7的免疫反应性。抗Leu 7和NSE在ENU诱导的神经肿瘤分类中价值不大。
