Turusov V S, Cabral J P
Cancer Research Centre, Russian Academy of Medical Sciences, Moscow.
Exp Toxicol Pathol. 1994 Oct;46(4-5):343-53. doi: 10.1016/S0940-2993(11)80114-4.
Twenty-five ethylnitrosourea (ENU) induced and 24 spontaneous tumours of peripheral nerves as well as 28 spontaneous mesenchymal tumours in BDVI rats were studied by light microscopy and immunohistochemically for the presence of S-100 protein (S-100). Early ENU-induced schwannomas*) representing the thickenings of nerves showed weak or negative S-100 immunoreactivity. S-100 positivity (both in the cytoplasm and nucleus) was observed in all large ENU-induced tumours found in animals dying or killed at advanced age. Immunostaining was present in both cystic and solid areas of schwannomas. S-100 positivity was found in 20 of 24 spontaneous schwannomas: 14 of 20 positive tumours contained cysts. Twenty-seven schwannomas (12 ENU-induced and 15 spontaneous) were studied for the presence of glial fibrillar acid protein (GFAP) and 13 were positive (7 had cystic areas). GFAP-positivity was relatively high in 4 tumours (3 ENU-induced and 1 spontaneous); these tumours also showed intense S-100 reactivity. Immunoreactivity for S-100 occurred more frequently and was much more intense than that for GFAP. The incidence of spontaneous peripheral nerve tumours in BDVI males reached 4%, cystic schwannomas being the most frequent type. All spontaneous mesenchymal tumours except lipoma (S-100 positive) were negative for S-100 protein and for GFAP.
对25只经乙基硝基脲（ENU）诱导及24只自发产生周围神经肿瘤的BDVI大鼠，以及28只自发产生间充质肿瘤的BDVI大鼠进行了光镜检查和免疫组织化学研究，以检测S-100蛋白（S-100）的存在情况。早期ENU诱导的神经鞘瘤*表现为神经增粗，S-100免疫反应性较弱或呈阴性。在老龄死亡或被处死动物体内发现的所有大型ENU诱导肿瘤中均观察到S-100阳性（细胞质和细胞核均阳性）。免疫染色在神经鞘瘤的囊性和实性区域均有出现。24只自发神经鞘瘤中有20只呈S-100阳性：20只阳性肿瘤中有14只含有囊肿。对27只神经鞘瘤（12只ENU诱导的和15只自发的）进行了胶质纤维酸性蛋白（GFAP）检测，其中13只呈阳性（7只含有囊性区域）。4只肿瘤（3只ENU诱导的和1只自发的）GFAP阳性率相对较高；这些肿瘤也表现出强烈的S-100反应性。S-100的免疫反应性比GFAP更频繁且更强烈。BDVI雄性大鼠自发周围神经肿瘤的发生率达4%，囊性神经鞘瘤是最常见的类型。除脂肪瘤（S-100阳性）外，所有自发间充质肿瘤的S-100蛋白和GFAP均为阴性。
*注：schwannomas原词有误，正确拼写应为schwannomas，即神经鞘瘤 。
