Nakopoulou L, Kerezoudi E, Thomaides T, Litsios B
Department of Pathology, University of Athens, Greece.
J Neurooncol. 1990 Feb;8(1):33-40. doi: 10.1007/BF00182084.
Immunostaining patterns of glial fibrillary acidic protein (GFAP), S-100 protein (S-100p) and vimentin were studied using immunohistochemical techniques on 48 paraffin embedded glial tumors. GFAP was positive in all tumor cases except in two oligodendrogliomas. S-100p was found in most astroglial tumors and in half of the oligodendrogliomas. Vimentin was positive in many astrocytomas but in no oligodendrogliomas. Most astroglial tumors showed similar immunoreactivity for GFAP and S-100p. Fibrillary processes, however, showed stronger and more crisp staining with anti-GFAP than with anti-S-100p, whereas cell nuclei were labeled only for S-100p. Vimentin was localised mainly in juxtanuclear positions. In many astrocytomas with different degrees of malignancy co-expression of GFAP, S-100p and vimentin was found. The presence of GFAP and S-100p was not correlated with the degree of differentiation in astrocytomas. Vimentin was more positive in anaplastic astrocytomas but this finding was not statistically significant. It seems that GFAP is a superior marker to S-100p and vimentin in the identification of human gliomas.
利用免疫组织化学技术，对48例石蜡包埋的神经胶质瘤进行了胶质纤维酸性蛋白（GFAP）、S-100蛋白（S-100p）和波形蛋白免疫染色模式的研究。除两例少突胶质细胞瘤外，所有肿瘤病例的GFAP均呈阳性。S-100p在大多数星形胶质细胞瘤和一半的少突胶质细胞瘤中被发现。波形蛋白在许多星形细胞瘤中呈阳性，但在少突胶质细胞瘤中均为阴性。大多数星形胶质细胞瘤对GFAP和S-100p显示出相似的免疫反应性。然而，纤维状突起用抗GFAP染色比用抗S-100p染色更强、更清晰，而细胞核仅被S-100p标记。波形蛋白主要定位于核周位置。在许多不同恶性程度的星形细胞瘤中发现了GFAP、S-100p和波形蛋白的共表达。GFAP和S-100p的存在与星形细胞瘤的分化程度无关。波形蛋白在间变性星形细胞瘤中更呈阳性，但这一发现无统计学意义。在人类胶质瘤的鉴别中，GFAP似乎是比S-100p和波形蛋白更好的标志物。
