Zhang Ming-Cui, Wang Qiong-E, Zhuang Hui-Sheng
College of Environmental Science and Engineering, Donghua University, Shanghai 201620, People's Republic of China.
Anal Bioanal Chem. 2006 Nov;386(5):1401-6. doi: 10.1007/s00216-006-0703-z. Epub 2006 Sep 1.
A novel, sensitive, and specific competitive fluorescence immunoassay has been developed for the quantitative determination of dibutyl phthalate (DBP) using an antibody-coated plate format. Hapten was synthesized in order to produce polyclonal antibodies against dibutyl phthalate. Polyclonal antisera to dibutyl phthalate were generated in rabbits and used to construct the fluorescence immunoassay for measurement of dibutylphthalate. The assay had a detection limit of about 0.02 microg L(-1), a dynamic range of approximately 0.1-300 microg L(-1). Other similar phthalate compounds do not interfere significantly in the analysis using this immunoassay technique, and the cross-reactivity rates were less than 10%. The study demonstrated that the developed antiserum and fluorescence immunoassay procedure can be used to detect dibutyl phthalate in environmental samples such as tap water, river water, drinking water, and leachate from plastic drinking water bottles.
已开发出一种新颖、灵敏且特异的竞争性荧光免疫分析法，用于采用抗体包被板形式定量测定邻苯二甲酸二丁酯（DBP）。合成了半抗原以制备抗邻苯二甲酸二丁酯的多克隆抗体。在兔体内产生了抗邻苯二甲酸二丁酯的多克隆抗血清，并用于构建测定邻苯二甲酸二丁酯的荧光免疫分析法。该分析法的检测限约为0.02 μg L⁻¹，动态范围约为0.1 - 300 μg L⁻¹。使用这种免疫分析技术进行分析时，其他类似的邻苯二甲酸酯化合物不会产生显著干扰，交叉反应率小于10%。研究表明，所开发的抗血清和荧光免疫分析方法可用于检测环境样品中的邻苯二甲酸二丁酯，如自来水、河水、饮用水以及塑料饮用水瓶的渗滤液。
