A. N. Bach Institute of Biochemistry, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia.
Biosensors (Basel). 2022 Nov 10;12(11):1002. doi: 10.3390/bios12111002.
The use of a large amount of toxic synthetic materials leads to an increase in the pollution of environmental objects. Phthalates are compounds structurally related to esters of phthalic acid that are widely used in the manufacturing of synthetic packaging materials as plasticizers. Their danger is conditioned by leaching into the environment and penetrating into living organisms with negative consequences and effects on various organs and tissues. This work presents the first development of lateral flow immunoassay to detect dibutyl phthalate, one of the most common representatives of the phthalates group. To form a test zone, a hapten-protein conjugate was synthesized, and gold nanoparticles conjugated with antibodies to dibutyl phthalate were used as a detecting conjugate. The work includes the preparation of immunoreagents, selectivity investigation, and the study of the characteristics of the medium providing a reliable optical signal. Under the selected conditions for the analysis, the detection limit was 33.4 ng/mL, and the working range of the determined concentrations was from 42.4 to 1500 ng/mL. Time of the assay-15 min. The developed technique was successfully applied to detect dibutyl phthalate in natural waters with recovery rates from 75 to 115%.
大量使用有毒合成材料会导致环境物体污染的增加。邻苯二甲酸酯是一种与邻苯二甲酸酯结构相关的化合物，广泛用于合成包装材料作为增塑剂。它们的危险条件是浸出到环境中，并渗透到具有负面后果和对各种器官和组织产生影响的生物体中。这项工作首次开发了侧向流动免疫分析来检测邻苯二甲酸二丁酯，这是邻苯二甲酸酯组中最常见的代表之一。为了形成测试区，合成了半抗原-蛋白质缀合物，并将与邻苯二甲酸二丁酯结合的金纳米粒子用作检测缀合物。这项工作包括免疫试剂的制备、选择性研究以及提供可靠光学信号的介质特性研究。在分析的选定条件下，检测限为 33.4ng/mL，测定浓度的工作范围为 42.4 至 1500ng/mL。测定时间为 15 分钟。该技术已成功应用于检测天然水中的邻苯二甲酸二丁酯，回收率为 75%至 115%。
