School of the Environment and Safety Engineering, Jiangsu University, Zhenjiang 212013, China.
School of Pharmacy, Jiangsu University, Zhenjiang 212013, China.
Talanta. 2018 Aug 15;186:104-109. doi: 10.1016/j.talanta.2018.04.023. Epub 2018 Apr 9.
A sensitive and artful colorimetric immunosensor based on horseradish peroxidase (HRP) was designed by labelling metal-organic frameworks (Cu-MOFs) on the second antibody (Cu-MOFs@Ab) as signal amplification for the detection of trace dibutyl phthalate (DBP). In this system, when Cu-MOFs@Ab was captured by antigen- primary antibody (Ab) complex, tremendous Cu(II) will be released from Cu-MOFs in the presence of nitric acid (HNO), and Cu(II) will be further reduced to Cu(I) after the addition of sodium ascorbate (SA), consequently, inhibiting the HRP to catalyse the colorless 3,3',5,5'-tetramethylbenzidine (TMB) into blue oxidized TMB (ox TMB). Under the optimized conditions, the limit of detection (LOD) was 1 μg L, which was almost 60 times lower than that using a conventional ELISA with the same antibody. In addition, our method showed good accuracy and reproducibility (recoveries of 87.73-103.4%; CV values of 1.46-5.95%) through a spike-recovery analysis. The proposed immunosensor indicated great potential for trace DBP determination from environmental and food samples.
基于辣根过氧化物酶（HRP）的灵敏且巧妙的比色免疫传感器，通过将金属有机骨架（Cu-MOFs）标记在第二抗体（Cu-MOFs@Ab）上，作为信号放大来检测痕量邻苯二甲酸二丁酯（DBP）。在该体系中，当 Cu-MOFs@Ab 被抗原-一抗（Ab）复合物捕获时，在存在硝酸（HNO）的情况下，大量的 Cu(II)将从 Cu-MOFs 中释放出来，并且在添加抗坏血酸钠（SA）后，Cu(II)将进一步还原为 Cu(I)，从而抑制 HRP 催化无色 3,3',5,5'-四甲基联苯胺（TMB）生成蓝色氧化 TMB（ox TMB）。在优化条件下，检测限（LOD）为 1μg L，比使用相同抗体的常规 ELISA 低约 60 倍。此外，通过加标回收分析，该方法显示出良好的准确性和重现性（回收率为 87.73-103.4%；CV 值为 1.46-5.95%）。该免疫传感器在环境和食品样品中痕量 DBP 的测定中具有很大的潜力。
