Handman E
Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
Infect Immun. 1990 Jul;58(7):2297-302. doi: 10.1128/iai.58.7.2297-2302.1990.
Leishmania major infection of macrophages is followed by a time-dependent appearance of lipophosphoglycan (LPG) that can be detected on the surface of infected cells by monoclonal antibodies. The origin of these LPG epitopes is probably the intracellular amastigote. LPG epitopes could be detected on the amastigote and the infected macrophage by a number of monoclonal antibodies directed to several distinct determinants on the phosphoglycan moiety. The macrophage-expressed LPG may be modified because, unlike the parasite LPG as expressed on promastigotes or amastigotes, it could not be radiolabeled by galactose oxidase or periodate treatment of infected cells followed by reduction with 3H-labeled sodium borohydride. Some LPG epitopes displayed on the macrophage may be anchored with glycosylphosphatidylinositol, and some may be in the water-soluble phosphoglycan form bound to macrophage integrins involved in its specific recognition. The water-soluble population could be released from the infected macrophage by gentle protease treatment.
巨噬细胞感染硕大利什曼原虫后，脂磷壁酸（LPG）会随时间出现，可通过单克隆抗体在感染细胞表面检测到。这些LPG表位的来源可能是细胞内无鞭毛体。通过多种针对磷酸聚糖部分几个不同决定簇的单克隆抗体，可在无鞭毛体和感染的巨噬细胞上检测到LPG表位。巨噬细胞表达的LPG可能经过了修饰，因为与前鞭毛体或无鞭毛体上表达的寄生虫LPG不同，对感染细胞进行半乳糖氧化酶或高碘酸盐处理，然后用3H标记的硼氢化钠还原，它无法被放射性标记。巨噬细胞上展示的一些LPG表位可能通过糖基磷脂酰肌醇锚定，一些可能以水溶性磷酸聚糖形式存在，与参与其特异性识别的巨噬细胞整合素结合。通过温和的蛋白酶处理，水溶性群体可从感染的巨噬细胞中释放出来。
