Moody S F, Handman E, Bacic A
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
Glycobiology. 1991 Sep;1(4):419-24. doi: 10.1093/glycob/1.4.419.
The lipophosphoglycan (LPG) of the intracellular amastigote form of the protozoan parasite Leishmania major is chemically distinct from the LPG on the surface of the extracellular promastigote form. Amastigote LPG is composed of the monosaccharides galactose, glucose, mannose, glucosamine and inositol in the molar ratio 51:30:24:1:1; arabinose is absent. The lipid anchor comprises four alkylglycerols, with alkyl chain lengths 24:0, 22:0, 20:0 and 26:0 in the molar ratio 68:18:8:6. Phosphate is present at 4% w/w of total carbohydrate. HPLC gel permeation reveals LPG to be a polydisperse family of molecules Mr 100-6 kDa. The results from immunological studies with LPG-directed antibodies are consistent with amastigote LPG having the expected tripartite structure of GPI-anchor, a core glycan and the phosphorylated disaccharide repeat backbone. Human sera from L. major patients bound amastigote LPG in enzyme-linked immunosorbent assays.
原生动物寄生虫硕大利什曼原虫细胞内无鞭毛体形式的脂磷壁酸（LPG）在化学组成上与细胞外前鞭毛体形式表面的LPG不同。无鞭毛体LPG由摩尔比为51:30:24:1:1的单糖半乳糖、葡萄糖、甘露糖、葡糖胺和肌醇组成；不含阿拉伯糖。脂质锚由四个烷基甘油组成，烷基链长度分别为24:0、22:0、20:0和26:0，摩尔比为68:18:8:6。磷酸盐占总碳水化合物的4%（w/w）。高效液相色谱凝胶渗透显示LPG是一个分子量为100 - 6 kDa的多分散分子家族。用LPG定向抗体进行的免疫学研究结果与无鞭毛体LPG具有预期的糖基磷脂酰肌醇锚、核心聚糖和磷酸化二糖重复主链的三方结构一致。来自硕大利什曼原虫患者的人血清在酶联免疫吸附测定中与无鞭毛体LPG结合。
