人皮肤利什曼原虫在小鼠巨噬细胞系中的研究：细胞内寄生虫的增殖与分离
Human cutaneous lieshmania in a mouse macrophage line: propagation and isolation of intracellular parasites.
作者信息
Chang K P
出版信息
Science. 1980 Sep 12;209(4462):1240-42. doi: 10.1126/science.7403880.
PMID:7403880
Abstract
A mouse macrophage line, J774G8, supports continuous and prolific intracellular growth of Leishmania mexicana amazonensis, the etiological agent of a South American cutaneous leishmaniasis. The intracellular parasites from these infected cultures can be isolated with high recovery rate and purity by simple Percoll gradient centrifugation.
摘要
一种小鼠巨噬细胞系J774G8能够支持墨西哥利什曼原虫亚马逊亚种（一种南美皮肤利什曼病的病原体）在细胞内持续大量生长。通过简单的Percoll梯度离心法，可从这些受感染培养物中分离出细胞内寄生虫，且回收率高、纯度高。
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