非洲凤凰木凝集素-2结合糖蛋白作为人结肠癌的一种新型碳水化合物抗原
Griffonia simplicifolia agglutinin-2-binding glycoprotein as a novel carbohydrate antigen of human colonic carcinoma.
作者信息
Nakayama J, Okano A, Maeda H, Miyachi M, Ota H, Katsuyama T, Kanai M
机构信息
Central Clinical Laboratories, Shinshu University Hospital, Matsumoto.
出版信息
Jpn J Cancer Res. 1990 Apr;81(4):388-95. doi: 10.1111/j.1349-7006.1990.tb02580.x.
Griffonia simplicifolia agglutinin-2-binding glycoprotein (GBG) in human colonic carcinoma was examined immunochemically and histochemically, GBG was extracted from colonic carcinoma as a serum-type glycoprotein of 160 kilodaltons. GBG was not identical with carcinoembryonic antigen (CEA), since its molecular weight and localization in tissue sections were different from those of CEA. The non-reducing terminals of GBG probably carry N-acetylglucosamine, but not blood group determinants. Furthermore, GBG was released by phosphatidylinositol-specific phospholipase C from cell membrane. GBG was suggested to be anchored to the membrane via linkage to a glycosyl-phosphatidylinositol molecule. Among colonic carcinoma-associated antigens, serum-type glycoproteins having N-acetylglucosamine at non-reducing terminals have not previously been reported. GBG is a novel carbohydrate antigen of human colonic carcinoma.
采用免疫化学和组织化学方法对人结肠癌中的西非吊灯树凝集素-2结合糖蛋白（GBG）进行了检测。GBG是从结肠癌中提取的一种160千道尔顿的血清型糖蛋白。GBG与癌胚抗原（CEA）不同，因为其分子量和在组织切片中的定位与CEA不同。GBG的非还原末端可能携带N-乙酰葡糖胺，但不携带血型决定簇。此外，GBG可被磷脂酰肌醇特异性磷脂酶C从细胞膜上释放出来。提示GBG通过与糖基磷脂酰肌醇分子连接而锚定在膜上。在结肠癌相关抗原中，此前尚未报道过在非还原末端具有N-乙酰葡糖胺的血清型糖蛋白。GBG是一种新型的人结肠癌碳水化合物抗原。
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