Toida M, Tsai C S, Okumura Y, Tatematsu N, Oka N
Department of Oral and Maxillo-Facial Surgery, Gifu University School of Medicine, Japan.
J Oral Pathol Med. 1990 Apr;19(4):155-9. doi: 10.1111/j.1600-0714.1990.tb00816.x.
The distribution of subunit A of Factor XIII (FXIIIa) and of collagenous components was investigated by the avidin-biotin-peroxidase complex (ABC) method for FXIIIa and by the Sirius red F3BA method, respectively, in 43 cases of radicular cysts. Besides the covering epithelial layer, the radicular cyst wall was composed of the following three layers: an inner granulomatous layer, an outer fibrous connective tissue layer, and an intermediate layer. In each layer, a positive reaction for FXIIIa was observed in certain connective tissue cells. These FXIIIa-containing cells were few in number in the inner layer where collagenous components were also sparse. In the slightly to moderately fibrous intermediate layer, these cells markedly increased in number and were dendritic or stellate in shape. In the outer densely fibrous connective tissue layer, they decreased slightly in number and were slender and spindle-shaped. The results obtained in the present study indicate the close relationship between the distribution of FXIIIa-containing cells and of collagenous components. Such a relationship suggests that these cells play an important role in the process of fibrosis occurring in the radicular cyst wall.
分别采用抗生物素蛋白-生物素-过氧化物酶复合物（ABC）法检测43例根端囊肿中凝血因子 XIII（FXIIIa）A 亚基的分布情况，并用天狼星红F3BA法检测胶原成分的分布情况。除覆盖上皮层外，根端囊肿壁由以下三层组成：内层为肉芽肿层，外层为纤维结缔组织层，中间为中间层。在每一层中，在某些结缔组织细胞中观察到FXIIIa呈阳性反应。这些含FXIIIa的细胞在内层数量很少，内层的胶原成分也很稀疏。在轻度至中度纤维化的中间层，这些细胞数量明显增加，呈树突状或星状。在外侧致密的纤维结缔组织层中，它们的数量略有减少，呈细长的纺锤形。本研究结果表明含FXIIIa的细胞分布与胶原成分分布之间存在密切关系。这种关系表明这些细胞在根端囊肿壁纤维化过程中起重要作用。
