Svec J, Hilubinová K, Lizorová V, Thurzo V
Neoplasma. 1975;22(2):133-45.
In contrast to the B77 virus, its B77(RBI) rat variant continuously produced by the rat RBI sarcoma cells in vivo and in vitro, efficiently induced progressively growing tumors in hamsters and transformed hamster embryo cells in vitro. Both, induced tumors and transformed cell cultures showed production of infectious virus and presence of the avian gs-antigen. Clonal analysis of the established hamster sarcoma cell line RBHtc brought evidence that despite the quantitative differences in the virus production and gs-antigen content among various clonal populations, full expression of the B77(RBI) virus genome does occur in each cell of the virus-induced hamster sarcoma. The permissive relationship of the B77(RBI) virus with rat and hamster cells as well as changed antigenic composititon implies that a genetic change of the virus occured, which is reflected in its increased mammalian tropism and its genetically stable firus-productive interaction with mammalian cells. The possible explanations of these phenomena are discussed.
与B77病毒不同，其B77(RBI)大鼠变体由大鼠RBI肉瘤细胞在体内和体外持续产生，能高效诱导仓鼠体内逐渐生长的肿瘤，并在体外转化仓鼠胚胎细胞。诱导的肿瘤和转化的细胞培养物均显示有传染性病毒产生且存在禽类gs抗原。对已建立的仓鼠肉瘤细胞系RBHtc进行的克隆分析表明，尽管不同克隆群体之间病毒产生量和gs抗原含量存在定量差异，但在病毒诱导的仓鼠肉瘤的每个细胞中确实都发生了B77(RBI)病毒基因组的完全表达。B77(RBI)病毒与大鼠和仓鼠细胞的许可关系以及抗原组成的变化意味着病毒发生了基因变化，这体现在其对哺乳动物嗜性的增加以及与哺乳动物细胞在基因上稳定的病毒生产性相互作用。文中讨论了这些现象的可能解释。
