Sabová L, Smida J, Smidová V
Neoplasma. 1983;30(6):659-66.
Injection of virogenic mouse cells B77-1026 into newborn Syrian hamsters resulted in arising of progressively growing autochthonous fibrosarcomas. From hamster tumors five stable tumor cell lines (BMH/1--BMH/5) were established in vitro. All cells of the newly established tumor cell lines had hamster karyotype, they were able to grow in soft agar and did not contain rescuable B77 viral genome. BMH tumor cells injected into syngeneic newborn as well as young adult hamsters produced tumors at the site of application and metastasized frequently into viscera. From metastases in different organs further tumor cell lines and single cell clones were established in vitro. All these tumor cell lines and clones exhibited higher metastatic capacity than the parent cell lines.
将致病毒的小鼠细胞B77 - 1026注射到新生叙利亚仓鼠体内，导致逐渐生长的自发纤维肉瘤出现。从仓鼠肿瘤中体外建立了五个稳定的肿瘤细胞系（BMH/1 - BMH/5）。新建立的肿瘤细胞系的所有细胞都具有仓鼠核型，它们能够在软琼脂中生长，并且不包含可拯救的B77病毒基因组。将BMH肿瘤细胞注射到同基因的新生以及年轻成年仓鼠中，在注射部位产生肿瘤，并经常转移到内脏。从不同器官的转移灶中体外进一步建立了肿瘤细胞系和单细胞克隆。所有这些肿瘤细胞系和克隆都表现出比亲本细胞系更高的转移能力。
