Sabová L, Smida J, Smidová V
Neoplasma. 1979;26(6):641-6.
Cells derived from a hamster tumor induced by avian sarcoma virus Bratislava 77 (B77) in vivo, were cultivated in vitro. After few passages two morphologically different cell lines were isolated from the parental culture (B77/H). One cell line consisted of fibroblastic cells (B77/H/fi and the other from epithelioid cells (B77/H/ep). Cells of both lines were highly tumorigenic in neonatal syngeneic hamsters, B77/H/ep cells were able to form colonies in soft agar and contained complete integrated B77 viral genome. In contrast, the B77/H/fi cells grew poorly in soft agar and did not contained B77 virus genome.
将源自禽肉瘤病毒布拉迪斯拉发77（B77）在体内诱导的仓鼠肿瘤的细胞进行体外培养。传代几次后，从亲代培养物（B77/H）中分离出两种形态不同的细胞系。一种细胞系由成纤维细胞组成（B77/H/fi），另一种由上皮样细胞组成（B77/H/ep）。两种细胞系的细胞在新生同基因仓鼠中均具有高度致瘤性，B77/H/ep细胞能够在软琼脂中形成集落，并含有完整整合的B77病毒基因组。相比之下，B77/H/fi细胞在软琼脂中生长较差，且不含B77病毒基因组。
