Winterhalter-Matas A
Znanstveni suradnik Imunoloskog zavoda u Zagrebu.
Lijec Vjesn. 1990 Jan-Feb;112(1-2):13-6.
Thermostability and propagation in different cell cultures and interferon-producing ability of three vaccine mumps virus strains (Jeryl Lynn, Leningrad-Zagreb and PZH-17) and ten wild viruses isolated from patients suffering from mumps were investigated. Considerable differences were found after vaccinal and wild virus strains were subjected to a temperature of 50 degrees C for 30 minutes. Wild strains were significantly more thermoresistant than the vaccinal ones. Differences in the propagation ability between wild an attenuated vaccinal strains were also found when tested in the Vero cell line and in primary cell cultures of human amnion. Wild strains propagated equally well in both cultures, while the vaccine strains propagated significantly better in the Vero cell line. The quantities of interferon produced by vaccinal and wild virus strains were determined by the micromethod titration technique. The vaccinal strains produced greater quantities of interferon than the wild ones, but from these differences no conclusions concerning the degree of their attenuation could be arrived at. From the results presented, it can be concluded that there is a correlation between the temperature marker and a difference in the propagation ability in the Vero cell cultures and the primary cell cultures of human amnion, and that these differences could be used as the genetic marker.
研究了三种腮腺炎疫苗病毒株（杰里尔·林恩株、列宁格勒-萨格勒布株和PZH-17株）以及从腮腺炎患者中分离出的十种野生病毒在不同细胞培养物中的热稳定性、增殖情况和产生干扰素的能力。将疫苗株和野生病毒株置于50摄氏度温度下30分钟后，发现了显著差异。野生株比疫苗株的耐热性明显更强。在Vero细胞系和人羊膜原代细胞培养物中进行测试时，还发现野生株和减毒疫苗株在增殖能力上存在差异。野生株在两种培养物中的增殖情况相同，而疫苗株在Vero细胞系中的增殖明显更好。通过微量滴定技术测定了疫苗株和野生病毒株产生的干扰素量。疫苗株产生的干扰素量比野生株多，但无法从这些差异得出关于其减毒程度的结论。从所呈现的结果可以得出结论，温度指标与在Vero细胞培养物和人羊膜原代细胞培养物中的增殖能力差异之间存在相关性，并且这些差异可作为遗传标记。
