Kashiwagi Y, Kawashima H, Takekuma K, Hoshika A, Mori T, Nakayama T
Department of Pediatrics, Tokyo Medical College, Japan.
J Med Virol. 1997 Jun;52(2):195-9.
A simple and sensitive method was developed for the differentiation of the Hoshino vaccine strain from wild strains with a restriction fragment length polymorphism (RFLP) analysis in the part of hemagglutinin-neuraminidase (HN) gene. The virus genome was amplified by using a reverse transcriptase-polymerase chain reaction (RT-PCR) directly from clinical samples. The PCR product of the Hoshino vaccine strain was cleaved into 2 fragments after digestion with Sca I and Afl II. All wild strains showed 2 RFLP profiles, A and B, different from that of vaccine strain. Wild A strains were cut into 2 fragments after digestion with Sca I only, while wild B strains were cleaved neither with Sca I nor Afl II. This molecular approach provides an effective method for differentiation of the Hoshino vaccine strain from wild strains of mumps virus in patients after vaccination.
开发了一种简单且灵敏的方法，通过对血凝素神经氨酸酶（HN）基因部分进行限制性片段长度多态性（RFLP）分析，来区分星野疫苗株和野生株。病毒基因组直接从临床样本中通过逆转录聚合酶链反应（RT-PCR）进行扩增。星野疫苗株的PCR产物在用Sca I和Afl II消化后被切割成2个片段。所有野生株显示出2种RFLP图谱，即A和B，与疫苗株的不同。野生A株在用Sca I消化后被切成2个片段，而野生B株既不被Sca I也不被Afl II切割。这种分子方法为区分接种疫苗后患者体内的星野疫苗株和腮腺炎病毒野生株提供了一种有效的方法。
