Castillo A, Razquin B E, Lopez-Fierro P, Alvarez F, Zapata A, Villena A J
Departamento de Biología Celular y Anatomía, Facultad de Biología, Universidad de León, Spain.
Thymus. 1990 May;15(3):153-66.
Owing to the lack of data about thymic non-lymphoid cells in fish we decided to perform a histochemical characterization of these cells in order to ascertain their relationships to other thymic components. In the present study we analyze the enzyme-histochemical patterns for acid phosphatase, alkaline phosphatase, non-specific sigma-naphthyl acetate esterase and 5' nucleotidase activities, as well as the presence of keratin demonstrated by immunoperoxidase staining, in the non-lymphoid cell populations of the thymus of the rainbow trout, Salmo gairdneri. According to their location in the organ, morphology and histochemical reactivities, we were able to define seven different subpopulations of keratin-positive epithelial cells: 1) Epithelial cells limiting with the capsular and septal connective tissues; 2) Subcapsular epithelial cells; 3) Stellate epithelial cells of the inner thymic zone; 4) Large, ovoid epithelial cells of the inner thymic zone; 5) Acidophilic epithelial cells of the outer thymic zone; 6) Cystic cells; and 7) Goblet cells. The significance of the heterogeneity of the epithelial cell (EC) population, its specific distribution in the organ, which apparently conforms distinct cell microenvironments, as well as the possible phylogenetical relationships between these microenvironments and the classical cortex and medulla of the mammalian thymus, are discussed.
由于缺乏鱼类胸腺非淋巴细胞的数据，我们决定对这些细胞进行组织化学特征分析，以确定它们与胸腺其他成分的关系。在本研究中，我们分析了虹鳟鱼（Salmo gairdneri）胸腺非淋巴细胞群体中酸性磷酸酶、碱性磷酸酶、非特异性α-萘乙酸酯酶和5'-核苷酸酶活性的酶组织化学模式，以及免疫过氧化物酶染色显示的角蛋白的存在情况。根据它们在器官中的位置、形态和组织化学反应性，我们能够定义七种不同的角蛋白阳性上皮细胞亚群：1）与被膜和间隔结缔组织相邻的上皮细胞；2）被膜下上皮细胞；3）胸腺内区的星状上皮细胞；4）胸腺内区的大的卵圆形上皮细胞；5）胸腺外区的嗜酸性上皮细胞；6）囊状细胞；7）杯状细胞。本文还讨论了上皮细胞（EC）群体异质性的意义、其在器官中的特定分布（这显然符合不同的细胞微环境），以及这些微环境与哺乳动物胸腺经典皮质和髓质之间可能的系统发育关系。
