Zucker Marjorie L, Murphy Carol A, Rachel Jane M, Martinez Gregory A, Abhyankar Sunil, McGuirk Joseph P, Reid Kimberly J, Plapp Fred V
Department of Pathology, Saint Luke's Hospital, Saint Luke's Regional Laboratories, 4401 Wornall Road, Kansas City, MO 64111, USA.
Lab Hematol. 2006;12(3):125-30. doi: 10.1532/LH96.06012.
The immature platelet fraction (IPF) as determined by the Sysmex XE-2100 is a rapid automated measure of the least mature component of the platelet population and is thought to correlate with thrombopoietic activity of the marrow. We investigated the ability of IPF to predict platelet recovery following hematopoietic progenitor cell (HPC) transplantation. IPF was compared to standard parameters of hematopoietic recovery, including the immature reticulocyte fraction (IRF), an early predictor of recovery. Fifty patients undergoing peripheral blood HPC transplantation (38 autologous and 12 allogeneic) were followed daily for 11 to 28 days after transplantation with measurement of IPF, IRF, absolute neutrophil counts (ANC) and platelet counts. Mean days to recovery for IPF was 3.1 days less than for platelet count (P <.0001), 3.8 days less than for ANC (P <.0001), and 0.6 days less than for IRF (P = .0477). IPF recovered at least 1 day prior to platelet count in 79% (38 of 48) of patients, and was followed by platelet count recovery within 1 to 12 days (mean, 4.1 days). When autologous and allogeneic patient groups were analyzed separately, IPF recovered significantly earlier than platelet count and ANC in both groups (P <.0001). Thrombopoietin (TPO) levels in 5 patients receiving transplants correlated with IPF; however, this appeared to be secondary to an inverse correlation of both TPO and IPF with platelet count. IPF is comparable to IRF as one of the earliest predictors of hematopoietic recovery following peripheral blood HPC transplantation. IPF could potentially be useful as a predictor of platelet recovery in other bone marrow failure syndromes.
Sysmex XE - 2100测定的未成熟血小板分数（IPF）是对血小板群体中最不成熟成分的一种快速自动化检测方法，被认为与骨髓的血小板生成活性相关。我们研究了IPF预测造血祖细胞（HPC）移植后血小板恢复的能力。将IPF与造血恢复的标准参数进行比较，包括未成熟网织红细胞分数（IRF），后者是恢复的早期预测指标。对50例接受外周血HPC移植的患者（38例自体移植和12例异体移植）在移植后每天进行随访，持续11至28天，期间检测IPF、IRF、绝对中性粒细胞计数（ANC）和血小板计数。IPF恢复的平均天数比血小板计数少3.1天（P <.0001），比ANC少3.8天（P <.0001），比IRF少0.6天（P =.0477）。在79%（48例中的38例）的患者中，IPF至少比血小板计数提前1天恢复，随后血小板计数在1至12天内恢复（平均4.1天）。当分别分析自体和异体患者组时，两组中IPF均比血小板计数和ANC恢复得明显更早（P <.0001）。5例接受移植患者的血小板生成素（TPO）水平与IPF相关；然而，这似乎是由于TPO和IPF与血小板计数呈负相关所致。作为外周血HPC移植后造血恢复的最早预测指标之一，IPF与IRF相当。IPF在其他骨髓衰竭综合征中可能作为血小板恢复的预测指标。
