Naz Arshi, Mukry Samina Naz, Shaikh Mahwish Rauf, Bukhari Ali Raza, Shamsi Tahir Sultan
Arshi Naz, Ph.D. National Institute of Blood Disease and Bone Marrow Transplantation, Karachi, Pakistan.
Samina Naz Mukry, Ph.D. National Institute of Blood Disease and Bone Marrow Transplantation, Karachi, Pakistan.
Pak J Med Sci. 2016 May-Jun;32(3):575-9. doi: 10.12669/pjms.323.9456.
Immune thrombocytopenic purpura (ITP) is a clinical syndrome in which a decreased number of circulating platelets (thrombocytopenia) manifests as a bleeding tendency, easy bruising (purpura) or extravasation of blood from capillaries into skin and mucous membranes (petechiae). The diagnosis of ITP can be made clinically on the basis of symptoms, we need to see if ITP can be confirmed in patients by quantification of residual RNA containing immature platelets (megakaryocytic mass) or immature platelets fraction (IPF) using automated hematology analyzers (Sysmex XE-2100).
In order to check the efficacy of IPF% parameter of Sysmex XE-2100 a total of 231 patients of thrombocytopenia were included in this study. Complete blood count (CBC) was estimated. The data was statistically analyzed by SPSS version 17.
About 62 patients were diagnosed as ITP and 169 patients were diagnosed as non ITP on the basis of clinical history. The mean IPF % value of ITP patients was 16.39% and the IPF % value of Non ITP patients was ~7.69% respectively. There was no significant difference in IPF% values with respect to time between sampling and acquisition of complete blood count. The diagnostic sensitivity of IPF% as biomarker for ITP and non-ITP was 85.71% (95%CI: 84.04% to 85.96%) and 41.76% (95% CI: 39.87% to 43.65%).
The mean IPF % value by Sysmex XE-2100 can be used to predict ITP.
免疫性血小板减少性紫癜（ITP）是一种临床综合征，循环血小板数量减少（血小板减少症）表现为出血倾向、易出现瘀斑（紫癜）或血液从毛细血管渗入皮肤和黏膜（瘀点）。ITP的诊断可基于症状进行临床判断，我们需要看看能否通过使用自动血液分析仪（Sysmex XE - 2100）对含有未成熟血小板的残留RNA（巨核细胞量）或未成熟血小板分数（IPF）进行定量，来在患者中确诊ITP。
为了检验Sysmex XE - 2100的IPF%参数的有效性，本研究纳入了总共231例血小板减少症患者。进行全血细胞计数（CBC）评估。数据采用SPSS 17版进行统计学分析。
根据临床病史，约62例患者被诊断为ITP，169例患者被诊断为非ITP。ITP患者的平均IPF%值为16.39%，非ITP患者的IPF%值约为7.69%。在采样与获取全血细胞计数之间的时间方面，IPF%值没有显著差异。IPF%作为ITP和非ITP生物标志物的诊断敏感性分别为85.71%（95%CI：84.04%至85.96%）和41.76%（95%CI：39.87%至43.65%）。
Sysmex XE - 2100测得的平均IPF%值可用于预测ITP。
