Lucchini A E, Lisa T A, Domenech C E
Departmento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
Arch Microbiol. 1990;153(6):596-9. doi: 10.1007/BF00245271.
In Pseudomonas aeruginosa and Rhizobium meliloti several choline derivatives, utilized as the sole carbon and nitrogen source, increase acid phosphatase activity. The enzyme activity of both bacteria could be released into the surrounding medium by EDTA-lysozyme treatment. The R. meliloti acid phosphatase activity of crude periplasmic extracts measured with p-nitrophenylphosphate was not inhibited by the presence of 5 mM choline, betaine, trimethylammonium or phosphorylcholine. The activity could not be detected using phosphorylethanolamine or phosphorylcholine as substrates. Among several phosphoesters tested only pyridoxal-5-phosphate was hydrolyzed at a considerable rate. In 7.5% polyacrylamide slab gel electrophoresis (non-denaturing conditions) of crude extracts obtained from bacteria grown in the presence of serine, glutamate, aspartate or dimethylglycine a phosphatase activity with identical mobility could be detected with alpha-naphthylphosphate or pyridoxal-5-phosphate were used as substrates. In conclusion, although the choline metabolites are capable of increasing acid phosphatase activities in R. meliloti and in P. aeruginosa, there are two different enzymes involved, apparently in different metabolisms.
在铜绿假单胞菌和苜蓿根瘤菌中，几种胆碱衍生物作为唯一的碳源和氮源被利用时，会增加酸性磷酸酶的活性。通过EDTA-溶菌酶处理，两种细菌的酶活性都可以释放到周围培养基中。用对硝基苯磷酸酯测定的粗周质提取物的苜蓿根瘤菌酸性磷酸酶活性不受5 mM胆碱、甜菜碱、三甲铵或磷酸胆碱的抑制。以磷酸乙醇胺或磷酸胆碱作为底物时无法检测到该活性。在测试的几种磷酸酯中，只有磷酸吡哆醛能以相当的速率被水解。在7.5%聚丙烯酰胺平板凝胶电泳（非变性条件）中，以α-萘基磷酸酯或磷酸吡哆醛为底物，从在丝氨酸、谷氨酸、天冬氨酸或二甲基甘氨酸存在下生长的细菌中获得的粗提取物中，可以检测到具有相同迁移率的磷酸酶活性。总之，尽管胆碱代谢产物能够增加苜蓿根瘤菌和铜绿假单胞菌中的酸性磷酸酶活性，但显然涉及两种不同的酶，它们参与不同的代谢过程。
