Garrido M N, Lisa T A, Albelo S, Lucchesi G I, Domenech C E
Departamento de Biología Molecular, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Córdoba, Argentina.
Mol Cell Biochem. 1990 Apr 18;94(1):89-95. doi: 10.1007/BF00223566.
Choline, betaine and N,N-dimethylglycine as the sole carbon and nitrogen source induced a periplasmic acid phosphatase activity in Pseudomonas aeruginosa. This enzyme produced the highest rates of hydrolysis in phosphorylcholine and phosphorylethanolamine among the various phosphoric esters tested. At saturating concentrations of Mg2+, the Km values were 0.2 and 0.7 mM for phosphorylcholine and phosphorylethanolamine respectively. At high concentrations both compounds were inhibitors of the enzyme activity. The Ksi values for phosphorylcholine and phosphorylethanolamine were 1.0 and 3.0 mM respectively. The higher catalytic efficiency was that of phosphorylcholine. Considering these results it is possible to suggest that the Pseudomonas aeruginosa acid phosphatase is a phosphorylcholine phosphatase. The existence of this activity which is induced jointly with phospholipase C by different choline metabolites, in a high phosphate medium, suggests that the attack of Pseudomonas aeruginosa on the cell host may also be produced under conditions of high phosphate concentrations, when the alkaline phosphatase is absent.
胆碱、甜菜碱和N，N-二甲基甘氨酸作为唯一的碳源和氮源可诱导铜绿假单胞菌产生周质酸性磷酸酶活性。在所测试的各种磷酸酯中，该酶对磷酸胆碱和磷酸乙醇胺的水解速率最高。在Mg2+饱和浓度下，磷酸胆碱和磷酸乙醇胺的Km值分别为0.2 mM和0.7 mM。在高浓度时，这两种化合物均为酶活性的抑制剂。磷酸胆碱和磷酸乙醇胺的Ksi值分别为1.0 mM和3.0 mM。磷酸胆碱的催化效率更高。考虑到这些结果，有可能认为铜绿假单胞菌酸性磷酸酶是一种磷酸胆碱磷酸酶。在高磷酸盐培养基中，这种由不同胆碱代谢物与磷脂酶C共同诱导的活性的存在表明，当碱性磷酸酶不存在时，铜绿假单胞菌对细胞宿主的攻击也可能在高磷酸盐浓度条件下发生。
