Biesterfeld Stefan, Beckers Sascha, Del Carmen Villa Cadenas Maria, Schramm Martin
Division of Cytopathology, Department of Pathology, Heinrich Heine University, Düsseldorf, Germany.
Anticancer Res. 2011 Jan;31(1):53-8.
The suitability of Papanicolaou staining and of hematoxylin staining for DNA single-cell cytometry was investigated in comparison to Feulgen staining.
Ten normal cervical smears and ten cervical smears containing cells of a squamous cell carcinoma in situ were analyzed. The integrated optical density (IOD) of 200 epithelial cells, chosen per random, was determined using a CM-1 TV-image analysis system (Hund, Wetzlar, Germany). Various DNA cytometric variables, accepted by the European Society for Analytical and Cellular Pathology (ESACP), and the mean nuclear area were calculated. Two measurements were performed after Papanicolaou staining (wavelengths: 530 nm and 590 nm), followed by measurements after hematoxylin re-staining (wavelength: 590 nm) and after Feulgen restaining (wavelength: 570 nm).
All histograms of Feulgen-stained normal squamous epithelia revealed a regular DNA distribution. The corresponding histograms after Papanicolaou staining or hematoxylin staining showed a wide scatter of values instead of a clear-cut diploid peak and an increased number of values >4c. Similar findings were observed in the carcinomatous smears. In particular, the mean values of the dispersion parameters (2cDI, entropy, ploidy imbalance and 2,5cEE) were significantly increased as compared to Feulgen staining.
Diagnostic or prognostic conclusions cannot be drawn from DNA measurements on Papanicolaou-stained or hematoxylin-stained specimens; Feulgen staining remains the gold standard for such purposes.
与福尔根染色相比，研究巴氏染色和苏木精染色用于DNA单细胞流式细胞术的适用性。
分析10份正常宫颈涂片和10份含有原位鳞状细胞癌细胞的宫颈涂片。使用CM - 1电视图像分析系统（德国韦茨拉尔的洪德公司）测定随机选取的200个上皮细胞的积分光密度（IOD）。计算欧洲分析与细胞病理学学会（ESACP）认可的各种DNA流式细胞术变量以及平均核面积。在巴氏染色后（波长：530 nm和590 nm）进行两次测量，随后在苏木精复染后（波长：590 nm）和福尔根复染后（波长：570 nm）进行测量。
福尔根染色的正常鳞状上皮的所有直方图均显示出规则的DNA分布。巴氏染色或苏木精染色后的相应直方图显示值的广泛分散，而不是清晰的二倍体峰，并且值>4c的数量增加。在癌性涂片中也观察到类似结果。特别是，与福尔根染色相比，离散参数（2cDI、熵、倍性失衡和2.5cEE）的平均值显著增加。
不能从巴氏染色或苏木精染色标本的DNA测量中得出诊断或预后结论；福尔根染色仍然是用于此类目的的金标准。
