Ishikawa K, Eguchi M, Sugiyama S, Iwama Y, Furukawa T, Hamaguchi H, Furusawa S, Shishido H
Second Department of Pediatrics, Dokkyo University School of Medicine, Tochigi-ken, Japan.
Exp Mol Pathol. 1990 Jun;52(3):259-65. doi: 10.1016/0014-4800(90)90067-n.
The ultrastructural localization of RNA in myeloma cells was studied by the RNase-gold method. Gold particles indicating the presence of RNA were observed in large numbers, particularly in the granular component of the nucleolus and periphery of the rough endoplasmic reticulum, but not in the Golgi area, mitochondria, intranuclear inclusion bodies, cytoplasmic inclusion bodies, dense bodies, or cisternae of the rough endoplasmic reticulum. In the nuclear chromatin and nucleolus, gold particles were more numerous as these structures were less mature. They were found in larger numbers also in the cytoplasm of immature cells. In plasma cells from patients with macroglobulinemia, gold particles were fewer than in myeloma cells of multiple myeloma, but there was no difference in their distribution pattern.
采用核糖核酸酶 - 金标法研究了骨髓瘤细胞中RNA的超微结构定位。观察到大量指示RNA存在的金颗粒，特别是在核仁的颗粒成分和粗面内质网的周边，但在高尔基体区域、线粒体、核内包涵体、胞质包涵体、致密体或粗面内质网的潴泡中未观察到。在核染色质和核仁中，随着这些结构成熟度降低，金颗粒数量更多。在未成熟细胞的细胞质中也发现有较多的金颗粒。在巨球蛋白血症患者的浆细胞中，金颗粒比多发性骨髓瘤的骨髓瘤细胞少，但它们的分布模式没有差异。
