Ishikawa K, Eguchi M
Second Department of Pediatrics, Dokkyo University School of Medicine, Japan.
Ultrastruct Pathol. 1989 Jul-Aug;13(4):405-12. doi: 10.3109/01913128909048491.
We analyzed semiquantitatively the ultrastructural distribution of RNA by the RNase-gold method in 40 patients with acute leukemia (20 patients with AML and 20 with ALL) before the initial treatment. The number of gold particles showing the presence of RNA was high in the granular component of the nucleolus and cytoplasm but low in the fibrillar component of the nucleolus, granules, the Golgi area, and Auer bodies. The number of gold particles in the nucleolus, nucleus, or cytoplasm was higher in AML than in ALL (p less than 0.01). The RNase-gold method seems to be useful for evaluating the capacity of protein synthesis, maturity, or differentiation of leukemic cells.
我们采用核糖核酸酶-金标法，对40例初治急性白血病患者（20例急性髓系白血病患者和20例急性淋巴细胞白血病患者）治疗前RNA的超微结构分布进行了半定量分析。显示RNA存在的金颗粒数量在核仁及细胞质的颗粒成分中较多，但在核仁的纤维成分、颗粒、高尔基体区域及Auer小体中较少。急性髓系白血病患者核仁、细胞核或细胞质中的金颗粒数量高于急性淋巴细胞白血病患者（p<0.01）。核糖核酸酶-金标法似乎有助于评估白血病细胞的蛋白质合成能力、成熟度或分化程度。
