通过组胺放射免疫测定法分析人外周血白细胞中的组胺释放。

Histamine release from human peripheral blood leukocytes analyzed by histamine radioimmunoassay.

作者信息

van Toorenenbergen A W, Vermeulen A M

机构信息

Laboratory of Allergology (Department of Clinical Chemistry), Erasmus University Hospital-Dijkzigt, Rotterdam, The Netherlands.

出版信息

Agents Actions. 1990 Apr;30(1-2):278-80. doi: 10.1007/BF01969060.

DOI:10.1007/BF01969060

PMID:1695450

Abstract

Histamine release from human peripheral blood leukocytes, after in vitro challenge with allergen extracts, is usually measured by fluorometry. In the present study we compared the results of the automated fluorometric histamine assay (Siraganian) with those of the Immunotech histamine radioimmunoassay. Histamine release dose-response curves obtained after histamine measurement by both methods were superimposable.

摘要

在体外用变应原提取物激发后，人外周血白细胞释放组胺的量通常用荧光测定法来测量。在本研究中，我们将自动荧光组胺测定法（西拉加尼安法）的结果与免疫技术组胺放射免疫测定法的结果进行了比较。用这两种方法测量组胺后得到的组胺释放剂量反应曲线是可叠加的。