一种用于检测人体体内和体外组胺释放的改良放射免疫测定法。
A modified radioimmunoassay for the detection of histamine release in vivo and in vitro in man.
作者信息
Assem E S, Osei D K
机构信息
Department of Pharmacology, University College London, UK.
出版信息
Agents Actions. 1990 Apr;30(1-2):287-90. doi: 10.1007/BF01969063.
Histamine (Hi) release in vitro or in vivo in man was measured by variants of a radioimmunoassay (RIA) procedure. Hi released from isolated basophils was converted enzymically to N-tau-methylhistamine (NMH) which was then measured by a very sensitive RIA. This modified RIA was compared with the standard spectrofluorometric assay and was found to have additional advantages in certain applications. RIA of NMH in plasma was found to be of value in acute medical conditions of obscure aetiology.
通过放射免疫测定法（RIA）的不同变体来测量人体体内或体外的组胺（Hi）释放量。从分离出的嗜碱性粒细胞释放的Hi经酶促转化为N-τ-甲基组胺（NMH），然后通过一种非常灵敏的RIA进行测量。将这种改良的RIA与标准荧光分光光度测定法进行比较，发现在某些应用中具有额外的优势。血浆中NMH的RIA在病因不明的急性病症中具有价值。
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