Morel A, Darmon M, Delaage M
Immunotech, Marseille, France.
Agents Actions. 1990 Apr;30(1-2):291-3. doi: 10.1007/BF01969064.
The clinical diagnosis of allergies needs appropriate tools for the measurement of histamine release from leukocyte suspension of affected patients. In the last few years the established fluorometric procedure has been challenged by alternatives like histamine radioimmunoassays (RIA). Although measurements of histamine with the latter methods make this procedure more accessible as a diagnostic tool, further simplifications are always welcomed. A newly developed immunoenzymoassay for histamine is described in the present work and compared to a recent radioimmunoassay. Sensitivity and specificity are shown to be comparable in both procedures.
过敏的临床诊断需要合适的工具来测量受影响患者白细胞悬液中组胺的释放。在过去几年中，已确立的荧光测定法受到了组胺放射免疫分析（RIA）等替代方法的挑战。尽管用后一种方法测量组胺使该程序作为诊断工具更容易获得，但进一步的简化总是受欢迎的。本文描述了一种新开发的组胺免疫酶测定法，并与最近的放射免疫分析法进行了比较。结果表明，两种方法的灵敏度和特异性相当。
