Goodyer P R, Langshur S, Fata J
Division of Pediatric Nephrology, Montreal Children's Hospital, Que., Canada.
Can J Physiol Pharmacol. 1990 Jun;68(6):733-6. doi: 10.1139/y90-111.
In mammalian kidney, epidermal growth factor (EGF) is produced as a small internal domain of an abundant high molecular weight peptide associated with the luminal membrane of the thick ascending limb of Henle's loop and distal convoluted tubule. At present, there is no evidence to indicate a mitogenic function for the EGF-containing molecule in kidney; consideration of its molecular structure suggests the possibility of a membrane-associated physiologic role. In this study, we examine regulation of renal EGF synthesis during induction of vitamin D deficiency in mice. Despite evidence of marked hyperparathyroidism, urinary excretion of EGF was equivalent in control (2.54 +/- 0.72 micrograms/mg creatinine) and vitamin D deficient (2.13 +/- 0.97 micrograms/mg creatinine) animals. Similarly, EGF mRNA levels in kidney were comparable in the two groups. These data indicate that parathyroid hormone has no effect on renal EGF regulation, although it is known to stimulate calcium reabsorption in distal nephron segments.
在哺乳动物肾脏中，表皮生长因子（EGF）是作为一种与髓袢升支粗段和远曲小管腔膜相关的丰富高分子量肽的小内部结构域产生的。目前，没有证据表明肾脏中含EGF的分子具有促有丝分裂功能；对其分子结构的研究表明它可能具有与膜相关的生理作用。在本研究中，我们检测了小鼠维生素D缺乏诱导过程中肾脏EGF合成的调节情况。尽管有明显的甲状旁腺功能亢进的证据，但对照组（2.54±0.72微克/毫克肌酐）和维生素D缺乏组（2.13±0.97微克/毫克肌酐）动物的EGF尿排泄量相当。同样，两组肾脏中的EGF mRNA水平也相当。这些数据表明，甲状旁腺激素对肾脏EGF调节没有影响，尽管已知它能刺激远端肾单位节段的钙重吸收。
