Salido E C, Lakshmanan J, Fisher D A, Shapiro L J, Barajas L
Department of Pathology, School of Medicine, University of California, Los Angeles, Harbor-UCLA Medical Center, Torrance 90509.
Histochemistry. 1991;96(1):65-72. doi: 10.1007/BF00266763.
The renal localization and the site of synthesis of epidermal growth factor (EGF) were investigated in the rat kidney by immunohistochemistry and in situ hybridization techniques. EGF was localized in the cells of the thick ascending limb of Henle (TAL) and distal convoluted tubule (DCT). At the ultrastructural level, EGF immunoreactivity was distributed on the apical membrane and trans-Golgi complex of the TAL and DCT cells. These segments of the rat nephron also hybridized to prepro-EGF cRNA probes in a specific manner, indicating that TAL and DCT are the sites of EGF synthesis in the rat kidney.
通过免疫组织化学和原位杂交技术，对大鼠肾脏中表皮生长因子（EGF）的肾定位及合成部位进行了研究。EGF定位于亨氏袢升支粗段（TAL）和远曲小管（DCT）的细胞中。在超微结构水平上，EGF免疫反应性分布于TAL和DCT细胞的顶端膜和反式高尔基体复合体上。大鼠肾单位的这些节段也以特异性方式与前EGF原cRNA探针杂交，表明TAL和DCT是大鼠肾脏中EGF的合成部位。
