Atkin B M, Franklin W A, Bell G I, Toback F G
Department of Medicine, University of Chicago, Ill.
Nephron. 1990;54(4):313-7. doi: 10.1159/000185886.
Epidermal growth factor (EGF) has previously been found in cells of the thick ascending limb of Henle's loop (TALH) and the first part of the distal convoluted tubule (DCT) of mouse kidney. To define the cellular localization of the mRNA that encodes the protein precursor of EGF, we used high-resolution in situ hybridization. EGF precursor cDNA was hybridized to both paraffin-embedded and frozen sections of mouse kidney. EGF precursor mRNA was detected in cells comprising the TALH and the first part of the DCT. In contrast, tubular cells in the macula densa did not express this specific mRNA. The results indicate that expression of the EGF precursor gene is restricted to specific types of cells in the distal nephron.
表皮生长因子（EGF）此前已在小鼠肾脏亨氏袢厚壁升支（TALH）和远曲小管起始段（DCT）的细胞中被发现。为了确定编码EGF蛋白前体的mRNA的细胞定位，我们采用了高分辨率原位杂交技术。将EGF前体cDNA与小鼠肾脏的石蜡包埋切片和冰冻切片进行杂交。在构成TALH和DCT起始段的细胞中检测到了EGF前体mRNA。相比之下，致密斑中的肾小管细胞不表达这种特定的mRNA。结果表明，EGF前体基因的表达仅限于远端肾单位的特定细胞类型。
