Klippmark E, Rydbeck R, Shibuta H, Norrby E
Department of Virology, Karolinska Institute, School of Medicine, Stockholm, Sweden.
J Gen Virol. 1990 Jul;71 ( Pt 7):1577-80. doi: 10.1099/0022-1317-71-7-1577.
Three human and six bovine parainfluenza virus type 3 (PIV3) strains were examined by the use of 60 monoclonal antibodies (MAbs). Fifty-three MAbs to the human C243 strain were directed against six, four, nine and seven epitopes of the haemagglutinin-neuraminidase (HN), fusion (F), nucleocapsid (N) and matrix proteins, respectively. Seven MAbs to the bovine strain were directed against three epitopes of the HN protein and three epitopes of the F protein. Each strain was characterized in ELISA and immunofluorescence tests with all MAbs and in a haemagglutination inhibition assay with the anti-HN MAbs. There were marked differences between human and bovine viruses, primarily in the HN protein where five epitopes differed. One epitope of the F and one of the N protein also differed. Bovine PIV3 was found to be a homogeneous subtype and distinct from human PIV3.
利用60种单克隆抗体（MAb）检测了3株人源和6株牛源3型副流感病毒（PIV3）。针对人C243株的53种MAb分别针对血凝素神经氨酸酶（HN）、融合蛋白（F）、核衣壳蛋白（N）和基质蛋白的6个、4个、9个和7个表位。针对牛源毒株的7种MAb分别针对HN蛋白的3个表位和F蛋白的3个表位。使用所有MAb通过ELISA和免疫荧光试验以及使用抗HN MAb通过血凝抑制试验对每种毒株进行了鉴定。人源和牛源病毒之间存在明显差异，主要在HN蛋白上，其中有5个表位不同。F蛋白的一个表位和N蛋白的一个表位也不同。发现牛PIV3是一个同源亚型，与人PIV3不同。
