Komada H, Tsurudome M, Ueda M, Nishio M, Bando H, Ito Y
Department of Microbiology, Mie University School of Medicine, Japan.
Virology. 1989 Jul;171(1):28-37. doi: 10.1016/0042-6822(89)90507-2.
Eighty monoclonal antibodies (MAbs) against parainfluenza virus type 4(PIV-4) were isolated and characterized. Of 50 MAbs against PIV-4A, 14 reacted with the nucleocapsid (NP) protein, 11 with the hemagglutinin-neuraminidase (HN) glycoprotein, 6 with the fusion (F) glycoprotein, and 19 with the matrix (M) protein. With the aid of the PIV-4A and PIV-2 specific MAbs showing cross-reactivity with PIV-4B, the structural proteins of PIV-4B were identified. gp72, p65, gp65, gp55, p53, and p40 of PIV-4B were assigned to HN, NP, Fo, F1, P, and M proteins, respectively. Based on the results, specificities of the MAbs against PIV-4B were determined. Of 30 hybridoma clones against PIV-4B, 13 clones were found to produce antibodies against the NP protein, 7 against the HN protein, and 10 against the F protein. Epitope mapping of these MAbs was performed with competitive binding assays in ELISA. According to their biological activities, the MAbs against the HN protein of either PIV-4A or 4B could be divided into three groups. The first group showed high hemagglutination inhibition (HI), hemolysis inhibition (HLI), and neutralizing (NT) activities. The second group showed high NT activity, but could not block hemagglutination. The final group showed a lower level of all activities. The MAbs against the F protein of PIV-4A and against PIV-4B were divided into two groups. Some MAbs against the F protein had high titer of NT, suggesting that the F protein had neutralizing-related epitopes. Antigenicity of the NP protein was highly conserved among subtypes of PIV-4. On the other hand, the MAbs against the HN and the F proteins showed high reactivity with the homologous subtype viruses, but low reactivity with the heterologous subtype viruses, indicating that the external glycoproteins exhibited antigenic variations between two subtypes of PIV-4. When the immunological interrelationship among various paramyxoviruses was analyzed. PIV-4 was found to be antigenically related to PIV-2, SV 5, and mumps virus.
分离并鉴定了80种抗4型副流感病毒（PIV-4）的单克隆抗体（MAb）。在针对PIV-4A的50种单克隆抗体中，14种与核衣壳（NP）蛋白反应，11种与血凝素神经氨酸酶（HN）糖蛋白反应，6种与融合（F）糖蛋白反应，19种与基质（M）蛋白反应。借助与PIV-4B具有交叉反应性的PIV-4A和PIV-2特异性单克隆抗体，鉴定了PIV-4B的结构蛋白。PIV-4B的gp72、p65、gp65、gp55、p53和p40分别对应于HN、NP、Fo、F1、P和M蛋白。根据这些结果，确定了抗PIV-4B单克隆抗体的特异性。在针对PIV-4B的30个杂交瘤克隆中，发现13个克隆产生抗NP蛋白的抗体，7个产生抗HN蛋白的抗体，10个产生抗F蛋白的抗体。通过ELISA中的竞争性结合试验对这些单克隆抗体进行了表位作图。根据其生物学活性，抗PIV-4A或4B的HN蛋白的单克隆抗体可分为三组。第一组表现出高血凝抑制（HI）、溶血抑制（HLI）和中和（NT）活性。第二组表现出高NT活性，但不能阻断血凝。最后一组的所有活性水平较低。抗PIV-4A的F蛋白和抗PIV-4B的单克隆抗体分为两组。一些抗F蛋白的单克隆抗体具有高滴度的NT，表明F蛋白具有与中和相关的表位。NP蛋白的抗原性在PIV-4的各亚型中高度保守。另一方面，抗HN和F蛋白的单克隆抗体与同源亚型病毒反应性高，但与异源亚型病毒反应性低，表明外部糖蛋白在PIV-4的两个亚型之间表现出抗原变异。当分析各种副粘病毒之间的免疫相互关系时，发现PIV-4与PIV-2、SV 5和腮腺炎病毒在抗原上相关。
