与罗斯氏菌属或直肠真杆菌相关的人类肠道优势菌群的分子多样性、培养及荧光原位杂交检测的改进
Molecular diversity, cultivation, and improved detection by fluorescent in situ hybridization of a dominant group of human gut bacteria related to Roseburia spp. or Eubacterium rectale.
作者信息
Aminov Rustam I, Walker Alan W, Duncan Sylvia H, Harmsen Hermie J M, Welling Gjalt W, Flint Harry J
机构信息
Gut Health Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, United Kingdom.
出版信息
Appl Environ Microbiol. 2006 Sep;72(9):6371-6. doi: 10.1128/AEM.00701-06.
Abstract
Phylogenetic analysis was used to compare 16S rRNA sequences from 19 cultured human gut strains of Roseburia and Eubacterium rectale with 356 related sequences derived from clone libraries. The cultured strains were found to represent five of the six phylotypes identified. A new oligonucleotide probe, Rrec584, and the previous group probe Rint623, when used in conjunction with a new helper oligonucleotide, each recognized an average of 7% of bacteria detected by the eubacterial probe Eub338 in feces from 10 healthy volunteers. Most of the diversity within this important group of butyrate-producing gut bacteria can apparently be retrieved through cultivation.
摘要
系统发育分析用于比较19株培养的人体肠道罗斯氏菌和直肠真杆菌菌株的16S rRNA序列与来自克隆文库的356条相关序列。发现培养的菌株代表了已鉴定的六个系统发育型中的五个。一种新的寡核苷酸探针Rrec584和先前的组探针Rint623，与一种新的辅助寡核苷酸一起使用时，在10名健康志愿者粪便中，它们各自识别的细菌平均占真细菌探针Eub338检测到的细菌的7%。这一重要的产丁酸肠道细菌组内的大多数多样性显然可以通过培养获得。
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