Duncan Sylvia H, Aminov Rustam I, Scott Karen P, Louis Petra, Stanton Thaddeus B, Flint Harry J
Gut Health Division, Rowett Research Institute, Greenburn Road, Bucksburn, Aberdeen AB21 9SB, UK.
National Animal Disease Center, USDA ARS, PO Box 70, 2300 Dayton Road, Ames, IA 50010, USA.
Int J Syst Evol Microbiol. 2006 Oct;56(Pt 10):2437-2441. doi: 10.1099/ijs.0.64098-0.
Seven recently cultured bacterial isolates, although similar in their 16S rRNA gene sequences to Roseburia intestinalis L1-82(T) (DSM 14610(T)), were not sufficiently related for inclusion within existing species, forming three separate clusters in a 16S rRNA gene phylogenetic tree. The isolates, which were obtained from human stools, were Gram-variable or Gram-negative, strictly anaerobic, slightly curved rods; cells from all strains measured approximately 0.5x1.5-5.0 mum and were motile. Two strains belonging to one cluster (A2-181 and A2-183(T)) were the only strains that were able to grow on glycerol and that failed to grow on any of the complex substrates tested (inulin, xylan and amylopectin). Strains belonging to a second cluster (represented by M6/1 and M72/1(T)) differed from the other isolates in their ability to grow on sorbitol. Isolates belonging to a third cluster (L1-83 and A2-194(T)) were the only strains that failed to grow on xylose and that gave good growth on inulin (strains M6/1 and M72/1(T) gave weak growth). All strains were net acetate utilizers. The DNA G+C contents of representative Roseburia strains A2-183(T), A2-194(T), M72/1(T) and R. intestinalis L1-82(T) were 47.4, 41.4, 42.0 and 42.6 mol%, respectively. Based on 16S rRNA gene sequence similarity, three novel Roseburia species are proposed, with the names Roseburia hominis sp. nov. (type strain A2-183(T)=DSM 16839(T)=NCIMB 14029(T)), Roseburia inulinivorans sp. nov. (type strain A2-194(T)=DSM 16841(T)=NCIMB 14030(T)) and Roseburia faecis sp. nov. (type strain M72/1(T)=DSM 16840(T)=NCIMB 14031(T)).
最近培养的7株细菌分离株，尽管其16S rRNA基因序列与肠道罗斯拜瑞氏菌L1-82(T)（DSM 14610(T)）相似，但亲缘关系不足以归入现有物种，在16S rRNA基因系统发育树中形成三个独立的簇。这些分离株从人类粪便中获得，革兰氏染色可变或革兰氏阴性，严格厌氧，略弯曲的杆菌；所有菌株的细胞大小约为0.5×1.5 - 5.0μm，具运动性。属于一个簇的两株菌（A2-181和A2-183(T)）是仅有的能够在甘油上生长而在任何测试的复合底物（菊粉、木聚糖和支链淀粉）上均不能生长的菌株。属于第二个簇的菌株（以M6/1和M72/1(T)为代表）在山梨醇上的生长能力与其他分离株不同。属于第三个簇的分离株（L1-83和A2-194(T)）是仅有的不能在木糖上生长而在菊粉上生长良好的菌株（菌株M6/1和M72/1(T)生长较弱）。所有菌株均利用乙酸盐净产乙酸。代表性罗斯拜瑞氏菌菌株A2-183(T)、A2-194(T)、M72/1(T)和肠道罗斯拜瑞氏菌L1-82(T)的DNA G+C含量分别为47.4、41.4、42.0和42.6 mol%。基于16S rRNA基因序列相似性，提出了三个新的罗斯拜瑞氏菌物种，分别命名为人类罗斯拜瑞氏菌（Roseburia hominis）新种（模式菌株A2-183(T)=DSM 16839(T)=NCIMB 14029(T)）；嗜菊粉罗斯拜瑞氏菌（Roseburia inulinivorans）新种（模式菌株A2-194(T)=DSM 16841(T)=NCIMB 14030(T)）；粪便罗斯拜瑞氏菌（Roseburia faecis）新种（模式菌株M72/1(T)=DSM 16840(T)=NCIMB 14031(T)）。
