初级微小RNA转录本的识别与切割

Recognition and cleavage of primary microRNA transcripts.

作者信息

Zeng Yan, Cullen Bryan R

机构信息

Department of Pharmacology, University of Minnesota, Minneapolis, MN, USA.

出版信息

Methods Mol Biol. 2006;342:49-56. doi: 10.1385/1-59745-123-1:49.

DOI:10.1385/1-59745-123-1:49

PMID:16957366

Abstract

MicroRNAs (miRNAs) are approx 22-nucleotide (nt)-long, single-stranded, endogenous, noncoding RNAs that are widely expressed in multicellular organisms. This chapter describes methods that allow the overexpression of human miRNAs and also discusses how primary miRNAs (pri-miRNAs), the much longer precursors of mature miRNAs, are processed in human cells, as well as in vitro.

摘要

微小RNA（miRNA）是长度约为22个核苷酸（nt）的单链内源性非编码RNA，在多细胞生物中广泛表达。本章介绍了实现人类miRNA过表达的方法，并讨论了初级miRNA（pri-miRNA），即成熟miRNA长得多的前体，在人类细胞以及体外是如何被加工的。