Balatsouka Dimitra, Gotfredsen Klaus, Sørensen Lars, Lindh Christian H, Berglundh Tord
Department of Prosthetic Dentistry, School of Dentistry, Health Science Faculty, University of Copenhagen, Norre Allé 20, 2200 CPH N, Copenhagen, Denmark.
Clin Oral Implants Res. 2006 Oct;17(5):488-94. doi: 10.1111/j.1600-0501.2005.01248.x.
The aim of the present study was to analyze the effect of systemic administration of nicotine on bone healing in osseous defects in the tibia of rabbits.
Sixteen female rabbits received nicotine (n=8; test group) or saline (n=8; control group) via subcutaneously placed mini-osmotic pumps for 8 weeks. The animals underwent three surgical operations during the experimental period, and body weight was registered weekly. Blood samples were collected to determine cotinine and prostaglandin E(2) levels. Bone preparations were made in the right leg of all rabbits after 4 weeks and in the left leg after 6 weeks of nicotine/placebo exposure. Thus, 2- and 4-week healing groups were created for the bone defects. After 8 weeks, the animals were killed. Tissue blocks including the bone defects were prepared for histological analysis.
The animals in the test group lost weight, while the control group gained weight during the experiment. The prostaglandin E(2) levels in plasma increased significantly following nicotine exposure in the test group. No significant differences in the percentage of vessels and bone density in the osseous defects were found between the test and the control groups after 2 and 4 weeks of healing.
In this experiment, systemic administration of nicotine over 4 or 6 weeks, respectively, influenced body weight and systemic prostaglandin E(2) levels but not the amount of blood vessels and the bone mineral density in bone defects after 2 or 4 weeks of healing.
本研究旨在分析全身给予尼古丁对兔胫骨骨缺损愈合的影响。
16只雌性兔通过皮下植入微型渗透泵分别接受尼古丁（n = 8；试验组）或生理盐水（n = 8；对照组）治疗8周。实验期间动物接受了三次外科手术，每周记录体重。采集血样以测定可替宁和前列腺素E2水平。在尼古丁/安慰剂暴露4周后对所有兔子的右腿进行骨标本制备，暴露6周后对左腿进行骨标本制备。因此，为骨缺损创建了2周和4周愈合组。8周后，处死动物。制备包括骨缺损的组织块用于组织学分析。
试验组动物在实验期间体重减轻，而对照组体重增加。试验组尼古丁暴露后血浆中前列腺素E2水平显著升高。愈合2周和4周后，试验组和对照组在骨缺损处的血管百分比和骨密度方面未发现显著差异。
在本实验中，分别全身给予尼古丁4周或6周，影响了体重和全身前列腺素E2水平，但在愈合2周或4周后，未影响骨缺损处的血管数量和骨矿物质密度。
