Department of Oral Rehabilitation, School of Dentistry, Health Science Faculty, University of Copenhagen, Denmark.
Department of Occupational and Environmental Medicine, Lund University Hospital, Lund, Sweden.
J Biomed Mater Res B Appl Biomater. 2009 Nov;91(2):918-923. doi: 10.1002/jbm.b.31475.
The aim of this study was to analyze the effect of longstanding nicotine exposure on bone healing and osseointegration of titanium implants.
20 female rabbits received either nicotine (n = 10) or saline (n = 10) administered subcutaneously via mini-osmotic pumps for 32 weeks. The pump delivered 6 microg/kg/min of nicotine for the animals in the test group. Blood samples were collected and plasma cotinine levels were measured monthly. Six months after the commencement of nicotine or saline administration three osteotomy preparations, one in right, femoral condyle and two in the right tibia were made. One experimental implant was placed in the femur site and one in the most distal preparation of the tibia. The remaining site in the tibia was left empty. The osteotomy preparation and implant installation procedure was repeated in the left leg of the rabbits after 2 weeks. The implants in the tibial site were subjected to removal torque test (RMT) 2 weeks later. Block biopsies of the two other osteotomy sites were prepared for histological analysis.
Although no differences in RMT values were found between test and control groups, there was a significant increase in RMT between 2 and 4 weeks within each group. The histomorphometric analysis of bone-to-implant contact and bone density in the bone defects revealed no differences between the test and the control group after 2 or 4 weeks of healing.
Longstanding (6 months) nicotine exposure did not impair bone healing and osseointegration of titanium implants.
本研究旨在分析长期尼古丁暴露对钛种植体骨愈合和骨整合的影响。
20 只雌性兔子接受了皮下注射迷你渗透泵的尼古丁（n = 10）或生理盐水（n = 10），持续 32 周。实验组动物的泵输送 6μg/kg/min 的尼古丁。每月采集血液样本并测量血浆可替宁水平。在开始给予尼古丁或生理盐水后的 6 个月，对右侧股骨髁和右侧胫骨的三个截骨部位进行了准备。一个实验植入物被放置在股骨部位，一个在胫骨的最远端准备部位。胫骨的剩余部位为空置。2 周后，在兔子的左腿重复进行截骨准备和植入物安装程序。2 周后，对胫骨部位的植入物进行了去除扭矩测试（RMT）。准备了另外两个截骨部位的块活检用于组织学分析。
尽管在 RMT 值上未发现实验组和对照组之间存在差异，但在每个组内，2 周和 4 周之间的 RMT 值有显著增加。在骨愈合的 2 周或 4 周后，对骨-种植体接触和骨密度的组织形态计量学分析显示实验组和对照组之间没有差异。
长期（6 个月）尼古丁暴露不会损害钛种植体的骨愈合和骨整合。
