Schubert Carsten J, Coolen Marco J L, Neretin Lev N, Schippers Axel, Abbas Ben, Durisch-Kaiser Edith, Wehrli Bernhard, Hopmans Ellen C, Damsté Jaap S Sinninghe, Wakeham Stuart, Kuypers Marcel M M
Department of Surface Waters, Eawag, Swiss Federal Institute of Aquatic Science and Technology, Seestrasse 79, CH-6047 Kastanienbaum, Switzerland.
Environ Microbiol. 2006 Oct;8(10):1844-56. doi: 10.1111/j.1462-2920.2006.01079.x.
Inputs of CH(4) from sediments, including methane seeps on the continental margin and methane-rich mud volcanoes on the abyssal plain, make the Black Sea the world's largest surface water reservoir of dissolved methane and drive a high rate of aerobic and anaerobic oxidation of methane in the water column. Here we present the first combined organic geochemical and molecular ecology data on a water column profile of the western Black Sea. We show that aerobic methanotrophs type I are responsible for methane oxidation in the oxic water column and ANME-1- and ANME-2-related organisms for anaerobic methane oxidation. The occurrence of methanotrophs type I cells in the anoxic zone suggests that inactive cells settle to deeper waters. Molecular and biomarker results suggest that a clear distinction between the occurrence of ANME-1- and ANME-2-related lineages exists, i.e. ANME-1-related organisms are responsible for anaerobic methane oxidation below 600 m water depth, whereas ANME-2-related organisms are responsible for this process in the anoxic water column above approximately 600 m water depth.
来自沉积物的甲烷输入，包括大陆边缘的甲烷冷泉和深海平原富含甲烷的泥火山，使黑海成为世界上溶解甲烷最大的地表水储存库，并驱动水柱中甲烷的高好氧和厌氧氧化速率。在此，我们展示了关于黑海西部水柱剖面的首个有机地球化学和分子生态学综合数据。我们表明，I型好氧甲烷氧化菌负责含氧水柱中的甲烷氧化，而与ANME-1和ANME-2相关的生物负责厌氧甲烷氧化。I型甲烷氧化菌细胞在缺氧区的出现表明无活性细胞沉降到更深的水域。分子和生物标志物结果表明，与ANME-1和ANME-2相关的谱系的出现存在明显区别，即与ANME-1相关的生物负责水深600米以下的厌氧甲烷氧化，而与ANME-2相关的生物负责在大约水深600米以上的缺氧水柱中的这一过程。
