Mel'nikov V G, Oleĭnik I I, Lerner L E
Lab Delo. 1990(5):68-70.
Fitness of various commercial nutrient media, manufactured in the USSR, for isolation and cultivation of fermentation-producing actinomyces was under study. The media were tested in two stages. Reference and newly isolated actinomyces cultures were examined at stage 1, clinical material at stage 2. Hottinger's blood agar was found the best for maintaining the growth of facultative anaerobic actinomyces and possessed the highest differentiating characteristics. This medium maintained sufficiently intensive growth of anaerobic actinomyces but only in the presence of meat extract. A liquid nutrient medium was designed, based on Hottinger's hydrolysate, that may be used for studies of these microorganisms' physiologic parameters.
对苏联生产的各种用于分离和培养产发酵放线菌的商业营养培养基的适用性进行了研究。这些培养基分两个阶段进行测试。第一阶段检测参考放线菌培养物和新分离的放线菌培养物，第二阶段检测临床材料。发现霍廷格血琼脂最适合维持兼性厌氧放线菌的生长，且具有最高的鉴别特性。该培养基能维持厌氧放线菌足够旺盛的生长，但仅在有肉浸液存在时才行。基于霍廷格水解物设计了一种液体营养培养基，可用于研究这些微生物的生理参数。
