Prieur A M, Granger G A
Transplantation. 1975 Oct;20(4):331-7. doi: 10.1097/00007890-197510000-00010.
The ability of concanavalin-A (Con-A) to activate lymphocytes to secrete human lymphotoxin (LT) was tested in the presence of agents known to modify the intracellular levels of cyclic nucleotides (cAMP and cGMP). Lymphocytes were treated with these agents at different stages of activation: (1) during the first encounter with mitogens, and (2) after they had been fully activated and were restimulated. Two agents, Dibutyryl cAMP and theophylline, dramatically inhibit the amount of LT secreted during both "primary" and "secondary" activation by Con-A. In contrast; DL-isoproterenol had a weak effect during primary activation, but greatly reduced the level of LT secreted during secondary activation. Agents which affect the intracellular level of cGMP were also tested. Imidazole had no effect on LT secretion by either primary or secondary Con-A activated cells. In contrast, carbamyl choline greatly reduced LT secretion to a level comparable to Dibutyryl cAMP and theophylline. All agents tested protected, to some degree, the sensitive alpha-L cell against LT-induced destruction in vitro. Agents which affect the levels of cyclic nucleotides affect both the effectiveness of the aggressor cell and the sensitivity of the target cells in vitro.
在已知可改变细胞内环核苷酸（cAMP和cGMP）水平的试剂存在的情况下，测试了伴刀豆球蛋白A（Con-A）激活淋巴细胞分泌人淋巴毒素（LT）的能力。在激活的不同阶段用这些试剂处理淋巴细胞：（1）在首次接触有丝分裂原期间，以及（2）在它们被完全激活并再次受到刺激之后。两种试剂，二丁酰cAMP和茶碱，显著抑制了Con-A在“初次”和“二次”激活期间分泌的LT量。相比之下，DL-异丙肾上腺素在初次激活期间作用较弱，但大大降低了二次激活期间分泌的LT水平。还测试了影响细胞内cGMP水平的试剂。咪唑对初次或二次Con-A激活细胞分泌LT均无影响。相比之下，氨甲酰胆碱将LT分泌大大降低至与二丁酰cAMP和茶碱相当的水平。所有测试的试剂在一定程度上保护了敏感的α-L细胞免受LT在体外诱导的破坏。影响环核苷酸水平的试剂在体外既影响攻击细胞的有效性，也影响靶细胞的敏感性。
