Rouveix B, Badenoch-Jones P, Larno S, Turk J L
Immunopharmacology. 1980 Dec;2(4):319-26. doi: 10.1016/0162-3109(80)90016-8.
The possible role of cyclic nucleotides in guinea pig macrophage aggregation, induced by human lymphokine (LK) has been investigated. Small increases were found in guanosine 3'5'-cyclic monophosphate (cGMP), but not adenosine 3'5'-cyclic monophosphate (cAMP), levels of lymphokine aggregated macrophages. Addition of exogenous dibutyryl (DB) cAMP, L-isoproterenol, or theophylline did not induce macrophage aggregation. By contrast, both exogenous DBcGMP and carbamyl-choline induced a macrophage aggregation; although DBcGMP was more effective. In addition, both L-isoproterenol and DBcAMP in the presence of theophylline decreased LK-induced macrophage aggregation, whereas D-isoproterenol and DBcGMP had no effect. The results obtained here are discussed in the context of the previously reported effects of cyclic nucleotides on migration inhibitory factor (MIF) activity and the possible role of these agents in the mechanism of action of MIF.
已经研究了环核苷酸在人淋巴因子（LK）诱导的豚鼠巨噬细胞聚集过程中可能发挥的作用。在淋巴因子聚集的巨噬细胞中，发现鸟苷3',5'-环磷酸（cGMP）水平有小幅升高，但腺苷3',5'-环磷酸（cAMP）水平没有变化。添加外源性二丁酰（DB）cAMP、L-异丙肾上腺素或茶碱不会诱导巨噬细胞聚集。相比之下，外源性DBcGMP和氨甲酰胆碱都能诱导巨噬细胞聚集；尽管DBcGMP的效果更明显。此外，在茶碱存在的情况下，L-异丙肾上腺素和DBcAMP都会降低LK诱导的巨噬细胞聚集，而D-异丙肾上腺素和DBcGMP则没有作用。本文所获结果结合先前报道的环核苷酸对迁移抑制因子（MIF）活性的影响以及这些物质在MIF作用机制中可能发挥的作用进行了讨论。
