Wisloff F, Christoffersen T
Int Arch Allergy Appl Immunol. 1977;53(1):42-9. doi: 10.1159/000231729.
Experiments were carried out in order to throw light on the role of cyclic AMP and cyclic GMP in the modulation of the expression of antibody-dependent cytotoxicity mediated by human peripheral blood lymphocytes in vitro. Chicken erythrocytes were used as target cells. Support for an inhibitory role of cyclic AMP was derived from the marked suppression of cytotoxicity by dibutyryl cyclic AMP. Cyclic AMP itself had little effect. Isoproterenol, prostaglandin E1 and prostaglandin E2 increased cyclic AMP concentrations and strongly inhibited the cytotoxic effect of the lymphocytes. Histamine showed very slight elevation of the cyclic AMP level and suppression of the cytotoxicity. Theophylline did not increase the cyclic AMP concentration, but potentiated the effects of isoproterenol and prostaglandin E1 on the cyclic AMP levels, and significantly inhibited cytotoxicity. No clear effects were seen when cyclic GMP, dibutyryl GMP, acetylcholine or carbacholine were added to the cells. The results indicate that in this system cyclic AMP inhibits cytotoxicity, while no evidence for a role of cyclic GMP has been obtained.
为了阐明环磷酸腺苷（cAMP）和环磷酸鸟苷（cGMP）在体外调节人外周血淋巴细胞介导的抗体依赖性细胞毒性表达中的作用，进行了实验。鸡红细胞用作靶细胞。二丁酰环磷酸腺苷对细胞毒性的显著抑制作用支持了环磷酸腺苷的抑制作用。环磷酸腺苷本身作用很小。异丙肾上腺素、前列腺素E1和前列腺素E2可提高环磷酸腺苷浓度，并强烈抑制淋巴细胞的细胞毒性作用。组胺使环磷酸腺苷水平略有升高并抑制细胞毒性。茶碱不会增加环磷酸腺苷浓度，但可增强异丙肾上腺素和前列腺素E1对环磷酸腺苷水平的作用，并显著抑制细胞毒性。当向细胞中添加环磷酸鸟苷、二丁酰环磷酸鸟苷、乙酰胆碱或卡巴胆碱时，未观察到明显作用。结果表明，在该系统中，环磷酸腺苷抑制细胞毒性，而未获得环磷酸鸟苷发挥作用的证据。
