Milner P, Crowe R, Kamm M A, Lennard-Jones J E, Burnstock G
Department of Anatomy, University College London, United Kingdom.
Gastroenterology. 1990 Sep;99(3):666-75. doi: 10.1016/0016-5085(90)90953-x.
The distribution in the bowel wall of vasoactive intestinal polypeptide-, neuropeptide Y-, and substance P-containing nerve cell bodies and nerve fibers has been described in human sigmoid colon by immunohistochemical examination. In patients with chronic idiopathic constipation, diverticular disease, and in controls (of tissue taken from patients with carcinoma, from a site distant from the tumor that appeared macroscopically normal), the concentrations of vasoactive intestinal polypeptide, neuropeptide Y, and substance P have been measured by immunoassay in the following preparations of sigmoid colon: mucosa, whole colonic wall with mucosa dissected away, circular muscle, and taenia coli. In idiopathic constipation, the vasoactive intestinal polypeptide content of the whole wall minus mucosa was reduced when compared with controls (P less than 0.05) but was unaltered in the mucosa, circular muscle, and taenia coli. In diverticular disease, the vasoactive intestinal polypeptide content of the mucosa and whole wall minus the mucosal layer was increased when compared with control tissue (P less than 0.05 and P less than 0.02, respectively) but was unaltered in the circular muscle and taenia coli. Substance P and neuropeptide Y levels in all layers of colonic wall were unaltered in these two diseases. The disturbances in the normal neural content of vasoactive intestinal polypeptide in the bowel wall in idiopathic constipation and diverticular disease may initiate or contribute to the functional changes seen in these disorders.
通过免疫组织化学检查，已描述了含血管活性肠肽、神经肽Y和P物质的神经细胞体及神经纤维在人乙状结肠肠壁中的分布。在慢性特发性便秘患者、憩室病患者以及对照组（取自癌症患者、距肿瘤远处且肉眼外观正常部位的组织）中，已通过免疫测定法在乙状结肠的以下制剂中测量了血管活性肠肽、神经肽Y和P物质的浓度：黏膜、去除黏膜的全结肠壁、环形肌和结肠带。在特发性便秘中，与对照组相比，去除黏膜后的全壁血管活性肠肽含量降低（P<0.05），但在黏膜、环形肌和结肠带中未改变。在憩室病中，与对照组织相比，黏膜和去除黏膜层后的全壁血管活性肠肽含量增加（分别为P<0.05和P<0.02），但在环形肌和结肠带中未改变。在这两种疾病中，结肠壁各层的P物质和神经肽Y水平均未改变。特发性便秘和憩室病中肠壁血管活性肠肽正常神经含量的紊乱可能引发或促成这些疾病中所见的功能变化。
