Jianshe Yang, Xigang Jing, Wenjian Li, Zhuanzi Wang, Guangming Zhou, Jufang Wang, Bingrong Dang, Qingxiang Gao, Linda Walsh
Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, 730000, China.
Radiat Environ Biophys. 2006 Nov;45(4):261-6. doi: 10.1007/s00411-006-0063-2. Epub 2006 Sep 9.
The biophysical characteristics of heavy ions make them a rational source of radiation for use in radiotherapy of malignant tumours. Prior to radiotherapy treatment, a therapeutic regimen must be precisely defined, and during this stage information on individual patient radiosensitivity would be of very great medical value. There are various methods to predict radiosensitivity, but some shortfalls are difficult to avoid. The present study investigated the induction of chromatid breaks in five different cell lines, including one normal liver cell line (L02), exposed to carbon ions accelerated by the heavy ion research facility in Lanzhou (HIRFL), using chemically induced premature chromosome condensation (PCC). Previous studies have reported the number of chromatid breaks to be linearly related to the radiation dose, but the relationship between cell survival and chromatid breaks is not clear. The major result of the present study is that cellular radiosensitivity, as measured by D0, is linearly correlated with the frequency of chromatid breaks per Gy in these five cell lines. We propose that PCC may be applied to predict radiosensitivity of tumour cells exposed to heavy ions.
