Mori Y, Fukuma K, Adachi Y, Shigeta K, Kannagi R, Tanaka H, Sakai M, Kuribayashi K, Uchino H, Masuda T
Department of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Gastroenterology. 1989 Aug;97(2):364-75. doi: 10.1016/0016-5085(89)90072-3.
Autoimmune gastritis accompanied by autoantibodies to parietal cells was induced in BALB/c nu/+ mice by neonatal thymectomy 2-4 days after birth. Three monoclonal autoantibodies, designated as 2B6 (IgG1), 2G10 (IgG2b), and 1H9 (IgG1), were obtained from one of these mice. All three reacted specifically with parietal cells, 2G10 recognizing species-specific antigenic determinants and 2B6 and 1H9 recognizing interspecies-specific antigenic determinants. All three recognized antigens on the membrane of intracellular secretory canaliculi and the cytoplasmic tubulovesicular system of parietal cells. At least two different molecular groups were recognized by these antibodies; 2B6 recognizing a 65,000-79,000-mol wt group and 1H9 recognizing a 92,000-120,000-mol wt group. Sera of most mice with autoimmune gastritis reacted with either or both groups. Both groups were consistently coprecipitated by any of the three antibodies when solubilized in NP-40. Sera, from patients with pernicious anemia, containing anti-parietal cell antibodies could also precipitate these two groups of antigens. Competition assay and physicochemical studies showed that the epitopes recognized by the three monoclonal antibodies are different.
出生后2 - 4天对BALB/c裸加小鼠进行新生期胸腺切除，诱导出伴有壁细胞自身抗体的自身免疫性胃炎。从其中一只小鼠中获得了三种单克隆自身抗体，分别命名为2B6（IgG1）、2G10（IgG2b）和1H9（IgG1）。这三种抗体均与壁细胞特异性反应，2G10识别种属特异性抗原决定簇，2B6和1H9识别种间特异性抗原决定簇。这三种抗体均识别壁细胞细胞内分泌小管膜和细胞质微管泡系统上的抗原。这些抗体识别至少两个不同的分子组；2B6识别分子量为65,000 - 79,000的组，1H9识别分子量为92,000 - 120,000的组。大多数患有自身免疫性胃炎的小鼠血清与其中一组或两组都发生反应。当溶解在NP - 40中时，这两组抗原均能被三种抗体中的任何一种持续共沉淀。含有抗壁细胞抗体的恶性贫血患者的血清也能沉淀这两组抗原。竞争试验和物理化学研究表明，三种单克隆抗体识别的表位不同。
